And the Spirit & the bride say, come.... Reveaaltion 22:17

And the Spirit & the bride say, come.... Reveaaltion 22:17
And the Spirit & the bride say, come...Revelation 22:17 - May We One Day Bow Down In The DUST At HIS FEET ...... {click on blog TITLE at top to refresh page}---QUESTION: ...when the Son of man cometh, shall he find faith on the earth? LUKE 18:8

Monday, August 27, 2012

Creation Moment 8/27/2012 - Venter's Experiment "create life"?- NO

But there is a God in heaven that revealeth secrets,
Daniel 2:28
http://www.sciencemag.org/content/329/5987/52.full.pdf
http://www.scienceagainstevolution.org/v14i10f.htm
"We did not create life from scratch: we transformed existing life into new life. Nor did we design and build a new chromosome from scratch. Rather, using only digitised information, we synthesised a modified version, a copy of the M. mycoides genome with 14 of its genes deleted and a "watermark" written in another 5000-plus base pairs. The result is not an "artificial" life form; it is a living, self-replicating cell that most microbiologists would find hard to distinguish from the progenitor cell, unless they sequenced its DNA.

It took 15 years to get to this proof-of-concept experiment. And it is just that: proof that it is possible to use a computer and four chemical bases to create a cell with no biological ancestor. Of course, we began by modifying an existing genome. Where else to start? Had we tried a new genome design it wouldn't have worked. Even so, we had 99 failures for every success.

Craig Venter
Complete genome assembly. In preparation for the final stage of assembly, it was necessary to isolate microgram quantities of each of the 11 second-stage assemblies. As reported, circular plasmids the size of our second-stage assemblies could be isolated from yeast spheroplasts after an alkaline-lysis procedure. To further purify the 11 assembly intermediates, they were exonuclease-treated and passed through an anion-exchange column. A small fraction of the total plasmid DNA (1/100th) was digested with Not I and analyzed by field-inversion gel electrophoresis (FIGE) (Fig. 2c). This method produced ~1 microgram of each assembly per 400 ml yeast culture (~1011 cells).

In plain English, here’s what they did: They needed to create a DNA molecule that was over 1 million base pairs long. (A DNA molecule is a twisted pair of long strings of four different kinds of molecules called bases. It looks like a twisted ladder. Each rung of the ladder is made from a pair of bases.)

They made this long DNA molecule by putting together smaller segments of commercially available DNA which they purchased from Blue Heron. They purchased pieces that were about 1,000 base pairs long and joined ten of them together to form pieces that were about 10,000 base pairs long. They took ten of these 10,000 base pair pieces and joined them together to form pieces about 100,000 base pairs long. Finally, they joined eleven of these 100,000 base pair pieces together to form the complete molecule.
What kind of a machine did they use to do this assembly, you might ask? They used living yeast, but it wasn’t easy." Discloure