"Investigators at U-M who are studying the nature of consciousness have successfully used the drug to identify the intricate brain geometry behind the unconscious state, offering an unprecedented look at brain structures that have traditionally been difficult to study.
A study published in the journal Nature Communications and led by Huang, George Mashour, M.D., Ph.D. and Anthony G. Hudetz, Ph.D., of the U-M Center for Consciousness Science, outlines for the first time in humans how the connections among brain cells within those two important areas are modified by propofol. The paper is titled "Propofol Disrupts the Functional Core-Matrix Architecture of the Thalamus in Humans."
In healthy volunteers, they mapped changes in the brain's architecture before, during and after propofol sedation, guided by functional magnetic resonance imaging (fMRI).
At baseline, explained Huang, the thalamus has a balanced level of activity of both specific nuclei (clusters of brain cells) that send sensory information to highly defined areas of the cortex—known as unimodal processing—and nonspecific nuclei that send information more diffusely throughout a higher layer of the cortex, known as transmodal processing.
The team found that, under deep sedation, the thalamus showed a drastic reduction in activity in clusters of brain cells responsible for transmodal processing, leading to a dominant unimodal pattern—suggesting that while sensory inputs are still received, there is no integration of those inputs.
Next, they discovered the specific cell types that played a role in the shift to an unconscious state and their relationship to the change in thalamic processing. The thalamus contains at least two distinct cell types, said Huang, core cells and matrix cells.
"We now have compelling evidence that the widespread connections of thalamic matrix cells with higher order cortex are critical for consciousness," says Hudetz, Professor of Anesthesiology at U-M and current director of the Center for Consciousness Science.
Imagining that the cortex is layered like an onion, core cells connect to lower layers while matrix cells connect to higher layers in a more spread-out manner.
By measuring mRNA expression signatures—like I.D. badges for the cells—they were able to see that a disruption in the activity of matrix cells played a greater role in the transition to unconsciousness than core cells. An additional surprise was that GABA, a major inhibitory transmitter in the brain usually thought to be key to propofol's actions, did not appear to play as prominent a role as expected.
"The results suggest that loss of consciousness during deep sedation is primarily associated with the functional disruption of matrix cells distributed throughout the thalamus," said Huang."
MedicalXpress
