1) Sin separates us from God (As Adam's sin did)
Or if his sin, wherein he hath sinned,
But your iniquities have separated between you and your God,
Isaiah 59:2
2) Sin separates us from our fellow man (As Cain's sin did)
And the LORD set a mark upon Cain,
Genesis 4:15
a kid of the goats, a male without blemish:
And he shall lay his hand upon the head of the goat,
and kill it in the place where they kill the burnt offering before the LORD:
it is a sin offering.
Leviticus 4:23,24