Monday, February 8, 2016

Creation Moment 2/9/2016 - Meet Crispr & Cas

Below is an article about the mechanics of YOUR DNA & how it can be used to repair diseases. All this follows patterns by figuring out how it works. Does anything else around you, with this level of organized complexity "evolve" from nothing to this systematic functioning? ...The answer is no..You would NEVER be dumb enough to apply Darwinian macro-evolution to a simple clock on the wall.
WHY apply the nonsense to something millions time more complex in it's ability of it's functionality?
For the invisible things of him from the creation of the world are clearly seen,
 being understood by the things that are made,
..........so that they are without excuse:
Romans 1:20

"Imagine a world where there is no cystic fibrosis, HIV or malaria; where crops are drought-resistant and parents no longer have to worry that their children may inherit a nasty disease. It might seem far-fetched – utopian even – but this is a potential reality thanks to a gene-editing technique that was discovered just three years ago.

The technique, known as Crispr (clustered regularly interspaced short palindromic repeats), is an advance on previous gene-editing techniques. In principle, it is more precise, faster, easier and cheaper, with the ability to delete, repair or replace genes. It’s considered such an upgrade that the scientific world, from human biology to agriculture, has jumped on the discovery with gusto.


DESIGN = DESIGNER {GOD}
It’s odd to think this all resulted from bacteria. There, scientists found DNA sequences that were repeated and interspersed with unique sequences, becoming known as Crispr. Further research identified the unique sequences as viral DNA, which had come from phage viruses that infect bacteria. The bacteria were essentially keeping a record of viral infection, forming part of their nifty microbial immune system. The other part was located near to the Crispr sequence; genes coding for enzymes known as Cas (Crispr-associated proteins), which have DNA-cleaving ability. 

The viral DNA sequences are copied into an RNA strand and linked with a Cas enzyme. The resulting molecules float through a bacterial cell on the lookout for viral DNA that specifically matches their RNA strands. A match results in the RNA and viral DNA pairing, allowing the Cas enzyme to whir into action and snip the viral DNA up, stopping the viral infection in its tracks.

This bacterial satnav and scissor combo has been exploited to become a genome-editor extraordinaire, targeting specific genes in any organism with the ability to delete, repair or replace them. All scientists need to do is synthesise a guide-RNA (gRNA) molecule of about 20 bases that matches the target gene sequence and link it to a Cas enzyme, of which there are many. The Cas enzyme most commonly used is Cas9 – from the bacteria that cause strep throat – because of its high efficiency and ability to create a double stranded DNA break. This gives rise to the Crispr–Cas9 nomenclature, though this system is often just referred to as Crispr

The gRNA–Cas9 complex then targets the specific gene to be edited through RNA–DNA base pairing and Cas9 makes the cut. One cut deactivates the gene. Two cuts, with two gRNAs, remove the gene. If the gene was faulty, it can then be repaired by adding a normal copy of the gene to the cell, which pairs up with the cut DNA ends to form one DNA molecule again. New genes can also be inserted into the genome in this way. Multiple gRNAs can also induce multiple cuts simultaneously, editing more than one gene at the same time. 

Correcting or replacing a dysfunctional gene can, in principle, result in normal gene expression and full correction of a disease,.." ChemistryWorld